Polymerase Chain Reaction Lab :: essays research papers

Title Polymerase Chain Reaction Simulation ProposeThe place of this lab was to understand how by running a mousse cataphoresis on a batch of DNA we are able to square off how many approximately cycles it has gone through.Methods Casting the Agarose Gel In this experiment .8% solvent was used. By using a 250ml flask the yellowish brown issue was prepared. Using the equation to make enough ancestor for the entire lab class the equation had to be multiplied by four. The contents of this equation were added to the 250ml flask and swirled to evenly distribute it contents. so a mark was move on the outside of the flask to indicate the level of the solution in the beginning heating. The flask opening had perafilm placed over it so that there was olive-sized to no evaporation. The solution was so placed in the microwave and heated. The solution was then heated for one min and swirled for evenly dissolved Agarose. The Agarose was then cooled, so that it was not to hot and the p late would crack. Some water was added to the solution because of there was some evaporation during heating. Once the gel had cooled, it was poured into the plate among the arctic dams. The plate was change about half way up the comb arms. These dams are placed in the plate to prevent leaking. Then the gel was added and allowed to completely soiditify, which takes around 20mins. Preparing the Gel for Electrophoresis once the rubber dams have been withdraw (carefully), the comb was then removed. Then the buffer was made. The buffer was made by using the equation, but also multiplying it by four, for the common chord lab groups. Then the chambers around the gel plate is filled with the buffer, just enough buffer to cover the gel plate in a very dainty amount. Then the dyes were loaded to there do wells. Once the gels were added (carefully) the lid was placed on the plate and system was moody on. The system ran for about 10mins. (Hint the system is running when there are bubble s occurring in the buffer solution.Once the gel had been run the exactly gel had been removed from the buffer, placed on tin foil and moisten with a small amount of buffer solution. Then the gel had a DNA Instastain tatter placed on authorise of it. The sheet was placed on the gel firmly and a beackr and gel casting tray were placed on top of the gel.